Cor(Cortisol) ELISA Kit

Product name:	Cor(Cortisol) ELISA Kit
Reactivity:	General
Alternative Names:	Hydrocortisone
Assay Type:	Competitive Inhibition
Sensitivity:	0.93 ng/mL
Standard:	200 ng/mL
Detection Range:	3.13-200 ng/mL
Sample type:	serum, plasma, urine and other biological fluids
Assay length:	2h
Research Area:	Metabolic pathway;Endocrinology;Hormone metabolism;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cor. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cor. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cor in the samples is then determined by comparing the OD of the samples to the standard curve.

Vendor	ELK Biotechnology CO.,Ltd. 鄂