scatter plot for cells with CD206 fitc on x-axis vs side scatter on y-axis

Anti-Hu CD206 Alexa Fluor® 488

SKU: A4-782-T100 Categories: แอนติบอดี, โฟลว์ ไซโตเมทรี, Vaccine

Description
Additional information

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody 15-2 (also known as MR15-2) recognizes an extracellular epitope of CD206 (macrophage mannose receptor, MMR), a 162-175 kDa type I transmembrane protein expressed mainly on macrophages, dendritic cells and hepatic or lymphatic endothelial cells, but not on monocytes.

Workshop

HLDA VII: WS Code 70802

Application

WB, FC (QC tested), ICC

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

Purified human mannose receptor

Preparation

Purified antibody is conjugated with Alexa Fluor^® 488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Description:

Our CD206 (Mannose Receptor) antibody is designed for reliable detection of CD206, a key marker expressed on macrophages, dendritic cells, and monocytes. This high-affinity antibody ensures accurate immunodetection in western blot (WB), flow cytometry (FC, QC tested), and immunocytochemistry (ICC) applications.

Key Features:
✔ High specificity for CD206 (MRC1)
✔ Validated for WB, FC (QC tested), and ICC
✔ Available in various conjugations (FITC, PE, Alexa Fluor®)
✔ Suitable for human, mouse, and rat samples

Applications:
🔬 Western Blot (WB) – Protein expression analysis
🧪 Flow Cytometry (FC, QC tested) – Cell surface marker detection
🦠 Immunocytochemistry (ICC) – Cellular localization studies