MEM Eagle w: EBSS, w/o: L-Glutamine, w: 2.2 g/L NaHCO3

MEM is an advancement of the BME and the base medium of many further modifications. Because BME did not fulfil all requirements for some mammalian and HeLa cells, a better variation had to be developed. Today, MEM is one of the most used synthetic media and shows its versatility by supplementing with amino acids including Hank`s or Earle`s salts. Even the addition of only small amounts of FBS results in a positive effect on cell growth.