Human Nanog Antibody, PE-conjugated

Description

Nanog is a member of the homeobox family of DNA binding transcription factors and has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF)/Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (Oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other’s expression level, which maintains the properties of ES cells.

Specification

Reactivity

Human

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Source

E. coli-derived recombinant human Nanog, Trp153-Val305, Accession # ABZ92376

Specificity

Detects human Nanog in direct ELISAs and Western Blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Nanog is observed.

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Application

Flow Cytometry

Full Name

Nanog Homeobox

Alternative Names

FLJ12581; FLJ40451; hNanog; homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox; Nanog

Entrez Gene IDs/Gene IDs

79923 (Human); 71950 (Mouse)

Endotoxin

<0.10 EU/μg by the LAL method.

Storage

At 2 – 8°C for one year. Protect from light.